Proteomics

Background

This is a new field of medicine and science which examines the proteins produced by different genetic sequences. By utilizing sophisticated measurements, pathologists are able to produce a protein fingerprint of various neoplasms, aiding in the diagnosis and prognosis of these tumors.

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SELDI AFFINITY MASS SPECTROMETRY

Proteomic Evaluation of Archival Cytologic Material Using SELDI Affinity Mass Spectrometry
Potential for Diagnostic Applications

Patricia A. Fetsch, MT(ASCP)
Nicole L. Simone
Peter K. Bryant-Greenwood, MD
Francesco M. Marincola, MD
Armando C. Filie, MD
Emmanuel F. Petricoin, PhD,
Lance A. Liotta, MD, PhD
and Andrea Abati, MD

Am J Clin Pathol 2002;118:870-876 Abstract quote

Proteomic studies of cells via surface-enhanced laser desorption/ionization spectrometry (SELDI) analysis have enabled rapid, reproducible protein profiling directly from crude samples.

We applied this technique to archival cytology material to determine whether distinct, reproducible protein fingerprints could be identified for potential diagnostic purposes in blinded specimens. Rapid Romanowsky–stained cytocentrifuged specimens from fine-needle aspirates of metastatic malignant melanoma (with both known cutaneous primary and unknown primary sites), clear cell sarcoma, and renal cell carcinoma and reactive effusions were examined using the SELDI technology. A unique characteristic fingerprint was identified for each disease entity. Fifteen "blinded" unknown samples then were analyzed.

When the protein profile fingerprints were plotted against the known fingerprints for the aforementioned diagnoses, the appropriate match or diagnosis was obtained in 13 (87%) of 15 cases. These preliminary findings suggest a substantial potential for SELDI applications to specific pathologic diagnoses.

Henry JB. Clinical Diagnosis and Management by Laboratory Methods. Twentieth Edition. WB Saunders. 2001.
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Sternberg S. Diagnostic Surgical Pathology. Third Edition. Lipincott Williams and Wilkins 1999.
Weedon D. Weedon's Skin Pathology. Second Edition. Churchill Livingstone. 2002.
Fitzpatrick's Dermatology in General Medicine. 5th Edition. McGraw-Hill. 1999.
Robbins Pathologic Basis of Disease. Sixth Edition. WB Saunders 1999.
Weiss SW and Goldblum JR. Enzinger and Weiss's Soft Tissue Tumors. Fourth Edition. Mosby 2001.
DeMay RM. The Art and Science of Cytopathology. Volume 1 and 2. ASCP Press. 1996.

Commonly Used Terms

Basic Principles of Disease
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Commonly Used Terms
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Diagnostic Process
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Surgical Pathology Report
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Last Updated 12/10/2002

LABORATORY/RADIOLOGIC/ OTHER TESTS	CHARACTERIZATION
LABORATORY MARKERS
SELDI AFFINITY MASS SPECTROMETRY
Proteomic Evaluation of Archival Cytologic Material Using SELDI Affinity Mass Spectrometry Potential for Diagnostic Applications Patricia A. Fetsch, MT(ASCP) Nicole L. Simone Peter K. Bryant-Greenwood, MD Francesco M. Marincola, MD Armando C. Filie, MD Emmanuel F. Petricoin, PhD, Lance A. Liotta, MD, PhD and Andrea Abati, MD	Am J Clin Pathol 2002;118:870-876 Abstract quote Proteomic studies of cells via surface-enhanced laser desorption/ionization spectrometry (SELDI) analysis have enabled rapid, reproducible protein profiling directly from crude samples. We applied this technique to archival cytology material to determine whether distinct, reproducible protein fingerprints could be identified for potential diagnostic purposes in blinded specimens. Rapid Romanowsky–stained cytocentrifuged specimens from fine-needle aspirates of metastatic malignant melanoma (with both known cutaneous primary and unknown primary sites), clear cell sarcoma, and renal cell carcinoma and reactive effusions were examined using the SELDI technology. A unique characteristic fingerprint was identified for each disease entity. Fifteen "blinded" unknown samples then were analyzed. When the protein profile fingerprints were plotted against the known fingerprints for the aforementioned diagnoses, the appropriate match or diagnosis was obtained in 13 (87%) of 15 cases. These preliminary findings suggest a substantial potential for SELDI applications to specific pathologic diagnoses.