Background
Hodgkin's disease has been divided into classic and non-classic types. This non-classic type also known as Nodular Lymphocyte Predominance has been separated from classic cases of Hodgkin's disease because of its distinct morphologic and clinical behavior. These patients usually present with isolated lymphadenopathy of several months duration. These patients have a clincal course resembling B cell Non-Hodgkin's lymphoma with a very indolent growth pattern with single and multiple relapses which may become generalized.
OUTLINE
HISTOLOGICAL TYPES CHARACTERIZATION GENERAL Diagnostic Reed-Sternberg cells are not required for diagnosis L and H cells Large cells with large nuclei and scant cytoplasm
Multilobated nuclear appearance resembling popcornLymph node Architecture focal obliterated with areas of uninvolved or hyperplastic lymphoid tissue-may have adjacent progressive transformation of germinal centers
Large nodules composed of the L and H cells are present imparting a mottled appearance to the node
Diffuse Lymphocyte Predominance (Diffuse paragranuloma) Cases which show minimal or absent nodularity but L and H cells present PROGRESSIVE TRANSFORMATION OF GERMINAL CENTERS (PTGC) Reactive follicular hyperplasia with enlargement of secondary follicles blurring the distinction between germinal centers and the mantle zone
Results in large nodules of small lymphocytes scattered among reactive follicles
Lacks L and H cells
Dark staining transformed follicles are 2-4x the size of surrounding follicles, lack well defined germinal centers or have irregular "broken-up" germinal centers
Formed mostly by small B lymphocytes, which are intermingled with isolated or small clusters of follicular center B cells
No polylobated L and H cells
May co-exist with Nodular lymphocyte predominant HD or precede its developmentRisk of developing Nodular lymphocyte predominant HD in a patient with a lymph node that shows a pure PTGC is low (0-5%)
VARIANTS
Characterization of variant patterns of nodular lymphocyte predominant hodgkin lymphoma with immunohistologic and clinical correlation.
Fan Z, Natkunam Y, Bair E, Tibshirani R, Warnke RA.
Am J Surg Pathol. 2003 Oct;27(10):1346-56. Abstract quote
SUMMARY: Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) has traditionally been recognized as having two morphologic patterns, nodular and diffuse, and the current WHO definition of NLPHL requires at least a partial nodular pattern. Variant patterns have not been well documented. We analyzed retrospectively the morphologic and immunophenotypic patterns of NLPHL from 118 patients (total of 137 biopsy samples). Histology plus antibodies directed against CD20, CD3, and CD21 were used to evaluate the immunoarchitecture.
We identified six distinct immunoarchitectural patterns in our cases of NLPHL: "classic" (B-cell-rich) nodular, serpiginous/interconnected nodular, nodular with prominent extranodular L&H cells, T-cell-rich nodular, diffuse with a T-cell-rich background (T-cell-rich B-cell lymphoma [TCRBCL]-like), and a (diffuse) B-cell-rich pattern. Small germinal centers within neoplastic nodules were found in approximately 15% of cases, a finding not previously emphasized in NLPHL. Prominent sclerosis was identified in approximately 20% of cases and was frequently seen in recurrent disease. Clinical follow-up was obtained on 56 patients, including 26 patients who had not had recurrence of disease and 30 patients who had recurrence. The follow-up period was 5 months to 16 years (median 2.5 years). The presence of a diffuse (TCRBCL-like) pattern was significantly more common in patients with recurrent disease than those without recurrence. Furthermore, the presence of a diffuse pattern (TCRBCL-like) was shown to be an independent predictor of recurrent disease (P = 0.00324). In addition, there is a tendency for progression to an increasingly more diffuse pattern over time.
Analysis of sequential biopsies from patients with recurrent disease suggests that the presence of prominent extranodular L&H cells might represent early evolution to a diffuse (TCRBCL-like) pattern. We also report three patients who presented initially with diffuse large B-cell lymphoma and later developed NLPHL.
SPECIAL STAINS/
IMMUNOPEROXIDASECHARACTERIZATION Classic immunophenotype for the L and H cells Show features consistent with B cell lineage:
CD19
CD20
CD22
CD74
CDw75
CD45RA
LCAGENERAL
Nodular lymphocyte predominant Hodgkin lymphoma. An immunophenotypic reappraisal based on a single-institution experience.Uherova P, Valdez R, Ross CW, Schnitzer B, Finn WG.
Department of Pathology, University of Michigan Medical School, Ann Arbor, USA.
Am J Clin Pathol 2003 Feb;119(2):192-8 Abstract quote In our experience, certain commonly cited immunophenotypic features of nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) are not encountered in day-to-day practice.
We reviewed 60 cases of NLPHL (18 women, 42 men; median age, 34 years) to discern immunophenotypic features from a large, single-institution cohort. All cases contained lymphocytic and histiocytic (L&H) cells. These cells expressed CD20 in 98% (59/60), CD79a (usually faint) in 87% (27/31), CD30 in 7% (4/59), epithelial membrane antigen in 21% (12/56), bcl-2 in 5% (2/41), and bcl-6 in 83% (30/36) of cases. CD10 was negative in all 36 cases studied; 100% of cases (55/55) demonstrated CD3+ rosettes. Although CD57+ T cells were common within the background infiltrate, CD57+ rosettes were seen in only 48% of cases (15/31) and were rare when encountered.
Based on these patterns, we conclude that bcl-2 and bcl-6 may be useful additions to the immunophenotypic analysis of NLPHL, but that the diagnostic usefulness of epithelial membrane antigen and CD57 rosettes may have been overemphasized in previous reports.
Small lymphocytes Mostly B cells Lymphocyte Predominance Hodgkin's Disease The Use of bcl-6 and CD57 in Diagnosis and Differential Diagnosis
Madeleine D. Kraus, M.D.; Jane Haley, HT, QIHC
From The Lauren V. Ackerman Laboratory of Pathology, Washington University School of Medicine, St. Louis, Missouri
Am J Surg Pathol 2000;24:1068-1078 Abstract quote
Distinction of lymphocyte predominance Hodgkin's disease (LPHD) from other forms of lymphoma often requires immunohistochemistry (IHC). Most previously published recommended panels include markers to define the large neoplastic cells (for example, CD20, J chain, CD45) as well as the non-neoplastic background cells (CD21, CD45RO, CD57, TiA1).
In the present study we examine the practical use of a double IHC method designed to look simultaneously at two germinal center specific cell types: bcl6+ cells and [bcl6+, CD57+] co-positive cells.
All 10 nodular LPHD had bcl6+ large cells and numerous CD57+ small background cells, including [bcl6+CD57+] cells in rosettes. One case of LPHD with large cell transformation contained numerous bcl6+ large cells both singly (in areas of typical LPHD) and in sheets (in foci of large cell transformation), many CD57+ small cells but few [bcl6+CD57+] co-positive cells and no rosettes. In none of the five cases of florid progressive transformation of germinal centers were true rosettes seen, although all contained variable numbers of bcl6+ large cells and CD57+ cells. Lymphocyte-rich classic Hodgkin's disease LRCHD cases were notable for bcl6 reactivity in Reed-Sternberg cells in all cases, numerous background small bcl6+ lymphocytes, and rare CD57+ cells. Two phenotypic profiles were associated with the 10 cases of T cell-rich B cell large cell lymphoma (TCRBCL). In the first, group ``A,'' six of six cases had bcl6– large cells and few CD57+ small cells, and none had significant numbers of [bcl6+, CD57+] co-positive cells. In the second, group ``B,'' four of four cases had bcl6+ large cells with numerous CD57+ and [bcl6+, CD57+] co-positive cells.
These findings not only show that LPHD can be distinguished from its morphologic mimics through identification of specific germinal center cell types, but also identifies a second group of TCRBCL (group ``B'') whose phenotype suggests it might be an architectural variant of nodular LPHD.
TARC, a CC Chemokine, Is Frequently Expressed in Classic Hodgkin's Lymphoma But Not in NLP Hodgkin's Lymphoma, T-Cell-Rich B-Cell Lymphoma, and Most Cases of Anaplastic Large Cell Lymphoma
S. C. Peh, M.B.B.S., F.R.C.Path.; L. H. Kim, B.BMed.Sc.; S. Poppema, M.D., Ph.D., F.R.C.P.
From the Department of Pathology, Faculty of Medicine, University of Malaya, Kuala Lumpur and Department of Pathology and Laboratory Medicine, University Hospital Groningen, The Netherlands.
Am J Surg Pathol 2001;25:925-929 Abstract quote
Thymus and activation-regulated chemokine (TARC) has been identified as a lymphocyte-directed CC chemokine that attracts activated T-helper type 2 (Th2) cells in humans. Recent studies showed that the T cells surrounding Reed-Sternberg cells in Hodgkin's lymphomas (HL) are Th2 type. Anaplastic large cell lymphomas (ALCL), T-cell-rich B-cell lymphoma (TCRBCL) can mimic HL in some instances.
This study aimed to establish the pattern of TARC expression in these diseases. Immunohistochemical stain using a polyclonal goat anti-human antibody to TARC was performed on 119 cases of confirmed HL; 99 were classical type (43 mixed cellularity, 43 nodular sclerosis, 5 lymphocyte depleted, 4 lymphocyte rich, 4 unclassifiable) and 20 lymphocyte predominant HL. Additional 27 ALCL (9 T-, 18 null-cell phenotype), 16 T-cell and 8 B-cell non-Hodgkin's lymphoma (NHL) were studied.
A total of 85.8% of the classical HL, one case of ALCL, and one case of large cell B-cell lymphoma with anaplastic morphology showed positive TARC expression in the tumor cells. The expression was paranuclear and/or diffuse in the cell cytoplasm. The tumor cells in all cases of lymphocyte predominant HL, TCRBCL, null ALCL, and T-NHL did not express TARC.
The high frequency of TARC expression in the Reed-Sternberg cells of classical HL may explain the characteristic T-cell infiltrate in this disease. The absence in other types that may be morphologically similar indicates that staining for TARC may aid in differential diagnosis.
DIFFERENTIAL DIAGNOSIS NODULAR LYMPHOCYTE-RICH CLASSICAL HODGKIN LYMPHOMA NODULAR LYMPHOCYTE PREDOMINANCE HODGKIN LYMPHOMA SIMILARITIES Sex M>F Same Mediastinal involvement Rare Same Stage Most commonly stage I/II (70-80%) Same B symptoms Rare Same Morphologic pattern Usually fairly closely packed large lymphoid nodules containing neoplastic large cells Same Immunophenotype of background cells Nodules composed of small B cells, punctuated by small cavities populated by the large neoplastic cells which are rosetted by T cells Same DIFFERENCES Age Slightly older (mean 43 years) Mean age 35 years Relapse pattern Relapses are usually single Repeated relapses, often delayed Treatment response at relapse Not good Good Clinical outcome Good (5 YRS 80%) but slightly worse than N-LPHL Very good (5YRS 98%) Morphological pattern of nodules Germinal centers commonly found in the nodules, and the neoplastic cells are concentrated in the expanded mantle zones Nodules do not have germinal centers
Neoplastic cells are concentrated within the nodules, but can also be found in the internodular areasCytology of neoplastic cells Reed-Sternberg cells and variants Popcorn (L and H) cells Immunophenotype of neoplastic cells CD20-/+, CD30+, CD15+/-, bcl-6- CD20+, CD30-, CD15-, bcl-6+ EBV association ~40% (but varies according to population) None
PROGNOSIS AND TREATMENT CHARACTERIZATION Prognostic Factors Stage is the most important factor
5 Year Survival 60% survival for stage IV disease
Transformation to a B or T cell Non-Hodgkin's lymphoma has been reported in 1-10% of cases
TREATMENT Dependent upon the clinical stage Atlas of Tumor Pathology-Tumors of the Lymph Nodes and Spleen. Volume 14, Third Series. AFIP Press. 1995.
Taken from Chan JKC. Practical Lymphoma Diagnosis: A Simplified Approach. Presented at the 111th Semi-Annual California Tumor Tissue Registry. December 2001.
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