Background
Examination of the bone marrow is one of the basic diagnostic examinations that a pathologist performs. Pathologists are often called upon to perform the procedure. In most cases, both a biopsy and an aspirate of the marrow is obtained. Aspirate samples are smeared on slides and usually stained with a Wright-Giemsa stain. Any remainder aspirate is allowed to clot and submitted for permanent paraffin sections. The biopsy is decalcified to soften the bone and then embedded in paraffin. Several elements are routinely analyzed in this examination.
OUTLINE
Gross Appearance and Clinical Variants Histopathological Features and Variants Special Stains/
Immunohistochemistry/
Electron MicroscopyCommonly Used Terms Internet Links
HISTOPATHOLOGY CHARACTERIZATION BONE MARROW ELEMENT ANALYZED EXPECTED FINDINGS Fat:Cell Ratio Varies by age of the patient
1st decade 10:90
Adult Varies from 30:70 to 70:30Myeloid:Erythroid Ratio Varies from 2.5:1 to 4:1 Myeloid Series See Table below Erythroid Series 15-37% of nucleated cells Megakaryocytes 0.5-2% of nucleated cells Lymphocytes and Lymphreticular Lesions 8-24% of nucleated cells
T:B cells 3:1Plasma Cells 3-6% of nucleated cells
Absent at birth
Normal immunogobulin ratio of kappa:lambda 4:1Metastatic Disease May require special stains for confirmation Reticulin and Collagen Proliferation Reticulin is reported as normal or increased, it usually preceedes collagen deposition Granulomatous Changes Nonspecific lipid granulomas are the most common type of granulomas and are not associated with infection
Other caseating and noncaseating granulomas require evaluation with special stains or cultures
Stainable Iron Stores Prussian blue stain highlights iron on biopsy and clot sections
Usually graded on a scale of 0-4 with 2-3 being adequate and 4-increasedMyeloid Precursors and Other Hematopoeitic Cells with Normal Range in Bone Marrow
Cell Type Range % Myeloblasts 0-2 Promyelocytes 2-5 Myelocytes (neutrophilic) 9-16 Metamyelocytes 7-23 Band forms 8-15 Neutrophils 4-10 Myelocytes (eosinophilic) 0-2 Band0-2 Mature0-3 Monocytes/macrophages 0-3 Basophils 0-1 Mast cells 0-2 Plasma cells 3-6
SPECIAL STAINS/
IMMUNOPEROXIDASE/
OTHERCHARACTERIZATION SPECIAL STAINS IMMUNOPEROXIDASE CD34
CD34/QBEND10 immunostaining in the bone marrow trephine biopsy: a study of CD34-positive mononuclear cells and megakaryocytes.Torlakovic G, Langholm R, Torlakovic E.
Department of Pathology, The Norwegian Radium Hospital, Oslo, Norway.
Arch Pathol Lab Med 2002 Jul;126(7):823-8 Abstract quote CONTEXT: The immunohistochemical detection of CD34 protein using QBEND10 antibody in bone marrow trephine biopsies was shown recently to be a precise method for quantitation of blasts and a possibly useful approach in diagnosis and classification of myelodysplastic syndrome.
OBJECTIVES: To evaluate CD34+ cells in bone marrow biopsies with various diagnoses and to assess how counts obtained using this method correlate with blast counts obtained by traditional morphologic evaluation of bone marrow smears. DESIGN: Bone marrow trephine biopsies from 108 adult patients were evaluated by immunohistochemistry using anti-CD34 antibody (QBEND10). CD34+ mononuclear cells were counted and compared with the blast counts in the bone marrow aspirate smears or imprints. CD34+ mononuclear cell clusters and CD34+ megakaryocytes were also recorded. The type of positivity (membranous vs cytoplasmic) and the percentage of CD34+ megakaryocytes were evaluated because the presence of CD34+ megakaryocytes was recently suggested to be present in myelodysplastic syndrome, but not in myeloproliferative disease or nonneoplastic bone marrow.
RESULTS: Six of 24 biopsies with partial involvement by non-Hodgkin lymphoma and 5 of 60 biopsies with reactive changes had 5% to 10% CD34+ mononuclear cells and were associated with lymphocytosis and increased hematogones. The CD34+ mononuclear cell clusters were found only in myelodysplastic syndrome and myeloproliferative disease. The CD34+ megakaryocytes were present in all diagnostic groups.
CONCLUSION: The number of CD34+ mononuclear cells was often slightly higher than the number of myeloid blasts in the bone marrow smears, probably due to increased hematogones. The presence and the number of CD34+ megakaryocytes do not appear to have diagnostic value, but this finding should be further investigated in relation to clinical parameters.
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Basic Principles of Disease
Learn the basic disease classifications of cancers, infections, and inflammation
Commonly Used Terms
This is a glossary of terms often found in a pathology report.Diagnostic Process
Learn how a pathologist makes a diagnosis using a microscopeSurgical Pathology Report
Examine an actual biopsy report to understand what each section meansSpecial Stains
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Pathologists actively oversee every area of the laboratory to ensure your report is accurateGot Path?
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Last Updated April 7, 2005
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